Kinetics of repair of DNA double-strand breaks under the influence of photon radiation

Batova A.S.1, Pilinskaya D.L., Dushanov E.B.1, Nasonova E.A.1, Bugay A.N.1

State University "Dubna", Russia, 141980, Dubna, st. University, 19, tel. 216-6081

1Joint Institute for Nuclear Research, Russia, 141980, Dubna, st. Joliot-Curie, 6, tel. 216-2119

The key damage to a cell that determines its fate is double-strand breaks (DR) of DNA, the incorrect repair of which leads to chromosomal aberrations that can be lethal for the cell [1]. Therefore, understanding the mechanisms of induction and kinetics of DNA DSB repair is important for predicting the radiation-induced response to radiation therapy and the survival of cells exposed to ionizing radiation.

This work is devoted to mathematical modeling of the DR repair process after exposure to rare ionizing radiation with different characteristics. Mathematical modeling of several stages of the cellular response to irradiation of Chinese hamster V79 cells with X-rays and human carcinoma cells with γ-radiation, such as the induction and repair of DNA DSBs, the kinetics of the formation of incorrectly repaired DNA double-strand breaks, and the formation of chromosomal aberrations, was carried out [2].

Comparative analysis of model data with experimental data obtained by the method of premature chromosome condensation [3] showed that for X-rays and for γ-rays, the kinetics of DR repair is the same in mammalian and human cells and obeys the same law. The number of DR and chromosomal aberrations is directly proportional to the radiation dose and the phase of the cell cycle.

The work was supported by the JINR AYSS grant (project no. 22-702-01).


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