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Conference publicationsAbstractsXXIX conferenceLinks between spatially extended models of astrocytic Ca2+ activity and analysis of Ca2+ imaging data: in search for synergyMV Lomonosov Moscow State University, fac. of Biology, Biophysics dep. 1 pp. (accepted)Astrocytes constitute an active milieu for the operation of networks of neurons, orchestrating the metabolic parameters of neural tissue and modulating synaptic connections. The astrocyte network divides the cortical space into non-overlapping domains, each encompassing hundreds of individual dendrites and hundreds of thousands of synapses. The bulk of our current knowledge about astrocyte function in the brain comes from calcium imaging employing genetically encoded calcium sensors or morphological imaging with pipette-loaded and genetically encoded reporters. The talk attempts to follow two converging paths: (1) Ca2+ dynamics imposed as the keystone and principal reporter of astrocyte activity is believed to be shaped and constrained by fine details of astrocyte morphology, which calls for frameworks of data-driven detailed simulations of Ca2+ dynamics; (2) Experimental Ca2+ imaging data are inherently noisy and require advanced analysis pipelines, but ground truth data needed for testing denoising and analysis is unavailable, while Ca2+ simulations can be used in a forward problem of generating fluorescent data where the ground truth is known.
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