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Conference publicationsAbstractsXX conferenceTextual analysis of experimantally proven and predicted promotersInstitute of Cell Biophysics RAS, Russia, 142290, Pushchino, Institutskaya str.3, phone: (4967)739319, E-mail: evgenia.teml@gmail.com 1 pp. (accepted)More than 1800 experimentally proven and predicted promoter regions available in RequlonDB were studied . The presence of -10 and -35 boxes, extended-10, oligoA-tracks, AT-rich rigions and spacer length were determined for each sequence. The AT-rich regions were additionally checked for similarity to consensus sequence, presented in the work . All textual elements (both boxes, extended-10 and UP-elements) were found only in 32 promoters from the whole dataset. About 300 promoters have extended-10 and more than 100 of them contain well shaped -35 box. A lot of promoter sequences has multiple sites satisfied the formal rules, so it was difficult to define precise location of textual elements. Such promoters, would benefit at the stage of initial recognition by RNAP because of higher probability of enzyme binding. The oligonucleotide composition of experimentally proven and predicted promoter sequences was additionally studied. It was shown that promoters with experimental evidence have apperent differences from computitionally predicted ones. References
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