|
Conference publicationsAbstractsXIX conferenceThe influences of pfenozan hybridization with alkyl residues or hydrophilic polymers on its interaction with biological membranesN.M.Emmanuel Institute of Biochemical Physics, Russian Academy of Sciences, Russia, 119334, Moscow, St.. Kosygin, 4. Tel. (495 ) 939-74-09, Fax (499)137-41-01, E-mail: olgavek@yandex 1 pp. (accepted)On the basis of antioxidant - pfenozan (- β - (4-hydroxy-3 ,5-di-tert-butylphenyl) propionic acid) in the ICP RAS were synthesized hybrid antioxidants ICHFAN and in the ICP RAS and S-P State. Univ. hybrid macromolecular antioxidants (GMAO) were synthesized . The hybridization determinate the target and the effective of the active substance - pfenozan, action. Charged onium group and a lipophilic long-chain alkyl tail in molecules of IHFANs allowed them to interact effectively with a charged lipid bilayer of the cell membranes and maintain the antioxidant status. In the molecules of GMAO hydrophilic polymers: hydroxyethyl starch and dextran, which covalently bound the phenols, allowed evenly distribute the active substance on the surface of the biomembrane. Comparison of the effects of pfenozan and its derivatives on the structural organization and function of components of animal cells revealed that, the pfenozan has a much milder impact, both on the membrane, and the whole cell, compared with IHFANs. Found a bimodal effect of ICHFAN to Ca2 +-dependent K + release out from erythrocytes, and small - of pfenozan. However, the morphology of erythrocytes pfenozan changes to a greater extent. ICHFANs concentrated in the membrane or in the protein with aid of the alkyl tails, that fact allowed us to reduce its concentration significantly . The presence of hydrophilic polymers in the molecules of GMAO "dilutes" phenols concentration, so the impact of GMAOs are weaker. And its are no formed the local concentrating of the active substance in the membrane or the protein due to a tenuous fixation of phenols.
|
